Simple Western Automated Western Blot Systems
เทคโนโลยี Simple Western™ นำเสนอการวิเคราะห์เวสเทิร์นแบบแคปิลลารีอัตโนมัติโดยใช้อิเล็กโทรโฟรีซิสแบบแคปิลลารีสำหรับการแยกโปรตีนตามด้วยการตรวจจับตามอิมมูโนแอสเซย์ภายในระบบที่ปิดสนิท เทคโนโลยี Simple Western ทำให้กระบวนการเวสเทิร์นบล็อตแบบดั้งเดิมง่ายขึ้นและเป็นระบบอัตโนมัติโดยผสานรวมเข้ากับระบบแคปิลลารีอัตโนมัติเต็มรูปแบบ ทำให้ได้ข้อมูลเชิงปริมาณที่แม่นยำกว่าเวสเทิร์นบล็อตแบบดั้งเดิม เนื่องจากมีเงื่อนไขการทำงานที่ควบคุมและเป็นมาตรฐาน
What is Simple Western?
Simple Western™ Technology provides automated capillary western analysis using capillary electrophoresis for protein separation followed by immunoassay-based detection within a fully enclosed system. Simple Western Technology simplifies and automates the traditional western blotting process by integrating it into a fully automated capillary system, providing more precise quantitative data than traditional western blotting due to its controlled and standardized operating conditions.
Simple Western is a fully automated western technology. Traditional western blotting steps of sample separation, wash buffer incubations, and immunodetection of target proteins occur inside a hands-free instrument on your benchtop.
Unlike traditional western blotting systems, Simple Western does not transfer proteins to a membrane, removing the inherent variability of western blotting and adding high-throughput sample processing and automated protein analysis.
Tiny amounts of sample are separated inside capillaries and are cross linked to the capillary walls. Then, blocking and wash buffer incubations are performed using just a few microliters of your primary antibodies and conjugated secondary antibodies to detect target proteins in chemiluminescence and NIR/IR fluorescence channels (LOD <1 pg).
Which Simple Western Instrument Is Right for You?
Size-Based Assays
Leo
The Leo™ System, powered by Simple Western™ Technology, is a high-throughput, fully automated capillary western platform. It enables fast, simple, and sensitive protein quantification and detection, processing up to 100 samples per run, increasing experimental size for quantitative assay designs and delivering results in as little as 3 hours.
Jess
Jess™ is a Simple Western™ instrument, an innovative technology from Bio-Techne, that performs capillary-based immunoassays and fully automated Western blot analysis, taking the complexity out of traditional immunoassays like Western blot and ELISA and filling in their shortcomings.
Abby is the next-generation chemiluminescence western system, designed to simplify your workflow and deliver publication-ready results with speed and reliability. With Abby, you can automate protein size separation and immunodetection for up to 25 samples, achieving analyzed results in just 3 hours. Experience picogram-level sensitivity with full automation, freeing you from the variability and time-consuming steps of traditional Western blots. Abby is the simple way to faster, error-free western results—making you never want to touch a manual blot again.
Abby
Sally Sue
Sally Sue™ lets you separate and analyze proteins by size from 2-440 kDa either by immunoassay or total protein. Got small sample volumes or starting materials? No problem. She uses as little as 0.2 µg/µL of protein in just 5 µL of sample, and runs up to 96 samples in one experiment
NanoPro 1000
NanoPro™ 1000 is a capillary-based nano-immunoassay platform, using capillary-based charge separation to characterize proteins. As in Western blot analysis, proteins from complex biological samples are separated, immobilized, and probed with antibodies. The NanoPro 1000 automates the entire process and uses isoelectric focusing (IEF) to resolve proteins.
Peggy Sue™ lets you separate and analyze proteins by size or charge from 2-440 kDa either by immunoassay or total protein. Got small sample volumes or starting materials? No problem. She uses as little as 0.2 µg/µL of protein in just 5 µL of sample, and runs up to 96 samples in one experiment.
Peggy Sue
